MicroVue OPG EIA

MicroVue OPG  EIA


The MicroVue OPG assay is an enzyme immunoassay for the quantitative measurement of total osteoprotegerin in plasma, serum, or other experimental sample types.



Catalog Number:   8034  
Format:   ELISA  
Tests/kit:   96 wells/40 (in duplicate)  
Sample Type:   Plasma, serum and experimental samples 

The Marker

Osteoprotegerin (OPG) or osteoclastogenesis inhibitory factor (OCIF) is a secretory glycoprotein belonging to the TNF super family. OPG consists of 401 amino acid residues; it has a molecular weight of 60 kDa as a monomer and 120 kDa as a disulfide-linked dimer and is produced in a wide range of tissues, e.g. bone, skin, liver, stomach, intestine, and lung. Osteoprotegerin inhibits the binding of RANK to RANKL (TRANCE, osteoprotegerin ligand, OPGL, osteoclast differentiation factor, ODF), thus inhibiting the recruitment, proliferation and activation of osteoclasts. Since OPG exhibits an inhibitory effect on osteoclasts, it acts as a soluble factor in the regulation of bone mass.1,2,3 Osteoclast formation activity may be the cause of bone loss in many imbalances in bone metabolism such as osteoporosis, osteopetrosis, hypercalcemia, metastatic osteolytic lesions and rheumatic bone degradation.2,4


Performance Characteristics


Method Sandwich
Analyte Osteoprotegerin
Specimen Plasma, Serum and experimental samples
Specimen Volume 100 μL
Limit of Detection 0.13 pmol/L
Dynamic Range 1.5-60 pmol/L
Precision CVs within-run 2.1-3.5%
Precision CVs between-run 4.2-6.1%
Incubation Time <3 Hours
Observed Values 5.7 ± 0.42 pmol/L
Species Reactivity Rhesus Macaque and Baboon. No reactivity detected with Cow, Chicken, Goat, Mouse, Rabbit, Rat, Sheep, Guinea Pig