MicroVue TRAP5b **
FOR RESEARCH USE ONLY IN THE UNITED STATES. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
The MicroVue TRAP5b Assay is an immunocapture enzyme assay for the determination of tartrate-resistant acid phosphatase isoform 5b (TRAcP 5b) in human serum and plasma.
Specifications 
8033 (Available in U.S. and Canada only)
8036 (IVD, CE Mark for EU and Customers Outside the U.S. and Canada) |
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ELISA |
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96 wells/ 40 (in duplicate) |
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Plasma and Serum |
The Marker
TRAP5b (serum band 5 tartrate-resistant acid phosphatase, TRAcP 5b; EC 3.1.3.2) is a 35-37 kDa glycoprotein. TRAP5b is typically expressed in proportion to osteoclast activity and is secreted into circulation. As a result, serum TRAP5b is considered a potentially useful marker for bone resorption. The MicroVue TRAP5b assay detects the enzyme activity of bound TRAP5b in experimental samples.
The Assay
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Format |
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96 well assay plate |
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Species Reactivity |
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Reactive with human derived TRAP5b only. No reactivity detected with Cow, Chicken, Goat, Mouse, Rabbit, Rat, Sheep, Guinea Pig, Baboon, Cynomolgus Monkey, Rhesus Macaque, |
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Assay Steps |
- Add 100 µL of Sample Diluent
- Add 50 µL of Calibrator, Controls and Specimens
- Seal the microplate; Incubate for 60 minutes at room temperature on a microplate shaker
- Wash the microplate wells three times with Wash Buffer
- Add 100 µL of Substrate Solution
- Seal the microplate; Incubate for 60 minutes at 37°C
- Add 50 µL of Stop Solution and Read OD at 405 nm
- Data Reduction Quadratic |