MicroVue Osteocalcin EIA
The MicroVue Osteocalcin immunoassay measures intact (de novo) osteocalcin in serum. Intact osteocalcin may be useful as a biochemical indicator of bone turnover.
Specifications 
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8002 |
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ELISA |
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96 wells/plate |
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Serum |
The Marker
Osteocalcin (OC) or BGP (bone gla protein) is found exclusively in bone tissue. It is a 5800 molecular weight extrahepatic vitamin K dependent protein produced by osteoblasts. It contains three gamma-carboxyglutamic acid residues which are thought to be involved in calcium ion and hydroxyapatite binding. It accounts for 10-20% of the noncollagenous protein in bone. While the in vivo function of osteocalcin is unknown, its affinity for bone mineral constituents implies a role in bone formation.
The Assay
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A Highly Specific Assay for Intact OC |
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Does not detect carboxy- or aminoterminal peptides
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Requires tertiary structure for antibody recognition
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Detects intact OC, not OC fragments released during osteoclastic resorption
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Format |
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96-well microplate with reagents sufficient to test 40 samples in duplicate |
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Species Reactivity |
- Rabbit, Pig, Sheep, Goat, Cow, Horse, Cynomolgus Monkey, Human Cell Culture, Guinea Pig
- No recognition of Rat, Mouse, or Dog |
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Assay Steps |
- Add 25 µL Standards, Controls, and Samples
- Add 125 µL anti-Osteocalcin Antibody
- Incubate 2 hours at room temperature
- Wash 3 times with 1X Wash Buffer
- Add 150 µL reconstituted Enzyme Conjugate
- Incubate 60 minutes at room temperature
- Wash 3 times with 1X Wash Buffer
- Add 150 µL Working Substrate Solution
- Incubate 35-40 minutes at room temperature
- Add 50 µL Stop Solution
- Read Optical Density at 405 nm
- Data Reduction: 4 Parameter |
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Performance Characteristics
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Competitive |
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Intact osteocalcin |
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Serum |
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25 µL |
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0.45 ng/ml |
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2 ng/ml |
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32 ng/ml |
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5-10% |
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5-10% |
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3.6 Hours |
- Intact OC 100%
- Reduced OC 0%
- N-, C- peptides 0% |