For In Vitro Diagnostic Use
MicroVue DPD is laboratory test that measures deoxypyridinoline cross-links (Dpd), a specific marker of bone resorption which is excreted in the urine. MicroVue DPD provides a quantitative measure of deoxypyridinoline (Dpd), a cross-link of bone type 1 collagen released during the resorption process. Dpd is excreted unmetabolized in urine. Elevated levels of urinary Dpd indicate elevated bone resorption in individuals.
Specifications 
|
8007 |
|
ELISA |
|
96 wells/plate |
|
Urine |
Intended Use
MicroVue DPD is intended for use as an aid in monitoring bone resorption changes in postmenopausal women receiving hormonal or biophosphonate antiresorptive therapies and in individuals diagnosed with osteoporosis.
The Assay
|
Format |
|
96-well microplate with reagents sufficient to test 40 samples in duplicate |
|
Species Reactivity |
|
Rat, Mouse, Rabbit, Guinea Pig, Dog, Pig, Sheep, Cow, Horse, Squirrel, Cynomolgus Monkey, Rhesus Macaque, Human Cell Culture |
|
Assay Steps |
- Add 50 µL of 1
- 10 diluted Standards, Controls and samples
- Add 100 µL cold Enzyme Conjugate
- Incubate 2 hours ± 5 minutes at 2-8°C in the dark
- Wash 3 times with 1X Wash Buffer
- Add 150 µL room temperature Working Substrate Solution
- Incubate 60 ± 5 minutes at room temperature
- Add 100 µL Stop Solution and read OD at 405 nm
- Data Reduction: 4 Parameter
- Correct for creatinine concentration |
|
|
Performance Characteristics
|
Competitive |
|
Deoxypyridinoline cross-links |
|
Urine |
|
50 µL |
|
1.1 nM/L |
|
3-300 nM |
|
4-8% |
|
3-5% |
|
3 Hours |
- Free Dpd 100%
- Free Pyd
- < 1%
- Pyd/Dpd peptides
- < 2.5% |
- Females
- 25-44 yr. premenopausal
- 3.0-7.4 nmol Dpd/mmol Cr
- Males
- 25-55 yr.
- 2.3-5.4 nmol Dpd/mmol Cr |
All Quidel assays are designed for laboratories equipped to perform microplate immunoassays as detailed on our Kit Format and Equipment Required page.