MicroVue Osteocalcin EIA

MicroVue Osteocalcin EIA

The MicroVue Osteocalcin immunoassay measures intact (de novo) osteocalcin in serum. Intact osteocalcin may be useful as a biochemical indicator of bone turnover.



Catalog Number:   8002  
Format:   ELISA  
Tests/kit:   96 wells/plate  
Sample Type:   Serum 

The Marker

Osteocalcin (OC) or BGP (bone gla protein) is found exclusively in bone tissue. It is a 5800 molecular weight extrahepatic vitamin K dependent protein produced by osteoblasts. It contains three gamma-carboxyglutamic acid residues which are thought to be involved in calcium ion and hydroxyapatite binding. It accounts for 10-20% of the noncollagenous protein in bone. While the in vivo function of osteocalcin is unknown, its affinity for bone mineral constituents implies a role in bone formation.


The Assay


A Highly Specific Assay for Intact OC
  • Does not detect carboxy- or aminoterminal peptides
  • Requires tertiary structure for antibody recognition
  • Detects intact OC, not OC fragments released during osteoclastic resorption
96-well microplate with reagents sufficient to test 40 samples in duplicate
Species Reactivity
- Rabbit, Pig, Sheep, Goat, Cow, Horse, Cynomolgus Monkey, Human Cell Culture, Guinea Pig
- No recognition of Rat, Mouse, or Dog
Assay Steps
- Add 25 µL Standards, Controls, and Samples
- Add 125 µL anti-Osteocalcin Antibody
- Incubate 2 hours at room temperature
- Wash 3 times with 1X Wash Buffer
- Add 150 µL reconstituted Enzyme Conjugate
- Incubate 60 minutes at room temperature
- Wash 3 times with 1X Wash Buffer
- Add 150 µL Working Substrate Solution
- Incubate 35-40 minutes at room temperature
- Add 50 µL Stop Solution
- Read Optical Density at 405 nm
- Data Reduction: 4 Parameter

Performance Characteristics


Method Competitive
Analyte Intact osteocalcin
Specimen Serum
Specimen Volume 25 µL
LOD* 0.45 ng/ml
LLOQ* 2 ng/ml
HLOQ* 32 ng/ml
Precision CVs within-run 5-10%
Precision CVs between-run 5-10%
Incubation Time 3.6 Hours
Cross-reactivity - Intact OC 100%
- Reduced OC 0%
- N-, C- peptides 0%