MicroVue BAP (Kemik Alkalen Fosfataz)

For In Vitro Diagnostic Use

MicroVue BAP provides a quantitative measure of bone-specific alkaline phosphatase (BAP) activity in serum. BAP is an enzyme produced by osteoblasts, the cells responsible for bone formation.

Specifications

 

Catalog Number:   8012  
Format:   ELISA  
Test/kit:   96 wells/plate  
Sample Type:   Serum 

Intended Use
MicroVue BAP is intended for use as an aid in the:
  • management of postmenopausal osteoporosis and Paget's disease;
  • monitoring of postmenopausal women on hormonal or biophosphonate therapy;
  • prediction of skeletal response to hormonal therapy in postmenopausal women.


 

The Assay

 

Format
96-well microplate with reagents sufficient to test 40 samples in duplicate
Species Reactivity
Rabbit, Pig, Dog, Sheep, Goat, Cow, Horse, Cynomolgus Monkey, Human Cell Culture. No recognition of Rat or Mouse
Assay Steps
- Add 125 µL Assay Buffer
- Add 20 µL Standards, Controls, and samples; swirl
- Incubate 3 hours ± 10 minutes at room temperature
- Wash 4 times with 1X Wash Buffer
- Add 150 µL room temperature Working Substrate Solution
- Incubate 30 ± 5 minutes at room temperature
- Add 100 µL Stop Solution and read OD at 405 nm
- Data Reduction: Quadratic curve
 

Performance Characteristics

 

Method Immuno-capture
Analyte Bone alkaline phosphatase
Specimen Serum
Specimen Volume 20 µL
Limit of Detection 0.7 U/L
Dynamic Range 2-140 U/L
Precision CVs within-run 4-6%
Precision CVs between-run 5-8%
Incubation Time 3.5 Hours
Cross-reactivity - Bone AP 100%
- Liver AP 3-8%
- Placenta AP 0%
- Intestine AP 0.4%
Expected Values - Females 25-44 yr. premenopausal
-  11.6 - 29.6 U/L
- Females ≥ 45 yr. postmenopausal
-  14.2 - 42.7 U/L
- Males ≥ 25 yr.
-  15.0 - 41.3 U/L

All Quidel assays are designed for laboratories equipped to perform microplate immunoassays as detailed on our Kit Format and Equipment Required page.
 

Medya